Kinesis Solutions Techtips
The latest Techtips from Kinesis Solutions.
- How to properly install sample loops
Stainless SteelStainless steel sample loops are supplied with fittings that are unswaged onto the tube. It is important that the loop be completely bottomed in the injector port before the ferrule is ...
More - Avoiding Pressure Transients
Air in the sample loop can cause instantaneous system pressure drop that eventually returns to a normal level. Air causes the pressure to drop when the injector moves from the ...More - How to properly install fittings
RheFlex® Stainless Steel (and titanium) A good square-cut, burr-free end on tubing is important for use in your system. The square-cut makes a flat surface for the tube to make co...More - Sample Loop Loading
Partial-FillingUse the partial-filling method if you need to conserve sample or if you want to vary sample volume frequently. In partial-filling, the syringe sets the volume injected onto the column. ...More - How to find and fix common leaks
Leaks cause valuable sample loss. Nobody wants that! The key to the valve holding pressure is the integrity of the sealing surfaces. If there is a scratch on the sealing surface or the needle seal in ...More - How to use proper syringe needles
With front-loading injectors, it is important to use the correct needle when loading the sample loop. An incorrect needle will damage the valve and can cause poor reproducibility. When the needle is t...More - How to Select the Right Rotor Seal
The standard rotor seal in many Rheodyne manual valves is made from a Vespel® blend. This polyimide has low wear and high chemical resistance. Vespel tolerates a pH range of 0 to 10. Solutions more ba...More - Burst Pressure of PEEK Tubing
PEEK tubing can be used instead of stainless steel tubing in most applications. PEEK is inert to almost all organic solvents and is biocompatible. Unlike metals, plastics are viscoelastic and, therefo...More - Fluidic Movement in Tubes
Q: "Why can I load only up to half of the volume of the loop in partial-filling method?"A: Sample occupies 2 µL of loop for every 1 µL loaded from the syringe. For example, 10 µL of sample spreads out...More - Achieving Low Levels of GC Column Bleed
What is column bleed?Column bleed is the normal background signal generated by the column stationary phase. This is illustrated in Figure 1 and it is easy to see from this why low column bleed is a si...More - GC Injection Techniques
When it comes to which injection technique is the best to use, there is no one correct answer due to the variety of samples and analyses required. No one method is optimal for all analyses, but in tod...More - Splitless Injection - Choosing the right conditions
Splitless injection is suitable for trace level determinations i.e. in situations where the analytes may be in the concentration range of low ppm, µg/mL. This allows much lower detection limits (highe...More - Choosing the right liner for the job
Welcome to the first of latest techtip from Kinesis intended to address the important considerations for inlet liner choice. If you have further questions on this or any other technical point, please ...More - The need to measure and optimise flow rate
Welcome to this months techtip from Kinesis Solutions "The need to measure and optimise flow rate". If you have any questions regarding this techtip or any others please contact us through our website...More - Choosing the right inlet supplies for trace analysis
When performing trace level analysis, particularly of active compounds such as certain pesticides, any source of activity can present major a problem. As can be seen (Figure 1), any activity in the li...More - Choosing the right right column
We are often asked for advice on selecting the right column, and among the characteristics that we ask people to consider are low bleed, a high maximum temperature limit, column inertness, and resista...More - Tips for Optimising Multiplier Lifetime
Electron Multipliers are extremely sensitive, high performance devices. Their working lifetime may be affected by a number of operational and system parameters such as handling techniques, operating g...More - GC Oven temperature and its effect on chromatography
When running a chromatogram there are 2 options for oven temperature. Either isothermally, which is defined as constant temperature for the entire chromatogram, or it can be a temperature programme. T...More - The Effect Of Liner Design
In previous techtips we have explained how using our FocusLiners can improve reproducibility, and how using a liner with a taper at the top can reduce flashback. However there are many other liner des...More
